Pyrosequencing-based barcodes for a dye-free multiplex bioassay.

نویسندگان

  • Zhiyao Chen
  • Xiaoying Fu
  • Xiaodan Zhang
  • Xiqun Liu
  • Bingjie Zou
  • Haiping Wu
  • Qinxin Song
  • Jinheng Li
  • Tomoharu Kajiyama
  • Hideki Kambara
  • Guohua Zhou
چکیده

A novel dye-free labeling method for a multiplex bioassay was proposed by using short sequence-based barcodes consisting of a reporter base and repeats of two stuffer bases; then, the barcodes were quantitatively decoded by a single pyrosequencing assay without any pre-separation.

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عنوان ژورنال:
  • Chemical communications

دوره 48 18  شماره 

صفحات  -

تاریخ انتشار 2012